Journal: Journal of translational medicine

Article Title: Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers.

doi: 10.1186/s12967-023-04438-3

Figure Lengend Snippet: Fig. 3 Differences between subsets of malignant cells with and without angiogenesis. A Single cell map showing eleven malignant cell subclusters. B UMAP plot of 14,151 cells from eight patient samples. C Tumor cell subclusters composition according to the patients. D Scatter plot showing the distribution of tumor cells in angiogenic and non-angiogenic breast cancer. E–F Violin plots and UMAP figures showing malignant cell-related markers and their distribution in malignant cell subgroups. G UMAP series of single-cell maps of BRCA_AGR3, BRCA_SRGN and BRCA_PPP1R1B. H Immunofluorescence imaging of AGR3, SRGN, PPP1R1B (green) and EpCAM (red) in three breast cancer tissue samples. The nuclei were counterstained with DAPI. Scale bar, 20 µm. I Representative images of the IHC of key genes between breast cancer and normal breast tissues available in the HPA database. J Volcano plot showing differences in gene expression genes in eleven tumor cell subclusters

Article Snippet: The secondary antibodies were subsequently added for 1 h at room temperature followed by counterstaining with DAPI (#AR1176, BOSTER).

Techniques: Immunofluorescence, Imaging, Gene Expression

Journal: Animals : an Open Access Journal from MDPI

Article Title: Vitamin A Supplementation Induces AMFK Production to Promote Cartilage Proliferation and Antler Growth in Sika Deer

doi: 10.3390/ani15192879

Figure Lengend Snippet: Effects of AMFK on chondrocyte proliferation and chondrogenic marker expression in vitro : ( A ) Cell proliferation analysis using the CCK-8 assay at different AMFK concentrations. ( B ) Representative images of EdU staining and DAPI counterstaining. ( C ) Quantification of EdU-positive cell percentage, **** p < 0.0001. ( D , E ) Immunofluorescence staining of SOX9 and COL2A1. ( F , G ) Fluorescence intensity quantification of SOX9 and COL2A1. ( H ) Relative mRNA expression levels of FGF9 , EDN1 , and SERPINE1 were analyzed by qPCR., * p < 0.05, *** p < 0.001, **** p < 0.0001. Representative fields from independent biological replicates (n ≥ 3).

Article Snippet: The slides were then incubated with the Alexa Fluor 488-conjugated secondary antibodies (SA00003-2, Proteintech, Wuhan, China) for 45 min, followed by nucleus staining with DAPI (4′6-diamidino-2-phenylindole, H-1200-10, Vectorlabs, Newark, CA, USA) for 5 min for nucleus labeling.

Techniques: Marker, Expressing, In Vitro, CCK-8 Assay, Staining, Immunofluorescence, Fluorescence